HOOFPRINT RED LAKE CRUISER “DIVA”
Sire: Hoofprint Itsanother Charly
Dam: Rin Tin Tins Sunflake
Owner: The Martin family.
Breeder: Joanne Chanyi
January 23, 1997 – January, 2007
9 years, 11 months
Yager-Best Histovet
Histological and cytological services
Suite A210, 49 York Road
Guelph, ON
N1E 6V1
Date received: 08-Mar-07
Date reported: 08-Mar-07
Case # YB 182121-3978-2007
Owner Name: Martin
Patient Name: Diva #1602 (Hoofprint Red Lake Cruiser)
Breed: German Shepherd
Sex: FS
Age: 10y (born Jan 23, 1997)
Tissue: multiple
There is mild autolysis present, particularly affecting the small intestinal samples.
In the stomach, there is moderate to severe inflammation throughout the
mucosa, and there is prominent mucosal lymphofollicular hyperplasia.
The inflammation consists of eosinophils, lymphocytes and plasma
cells, and rare neutrophils can also be seen. The gastric glands
are tightly packed and the gland mass is adequate. In the small
intestine, the tips of the villi are autolysed and cannot be assessed.
In the deeper mucosa, the crypts are tightly packed and the
cellularity of the lamina propria is normal. We do not see
submucosal infiltrates. The colon is normal. The glands are
tightly packed, the surface epithelium is intact and mature and the
cellularity of the lamina propria is normal.
In the kidney, there is mild thickening of the glomerular mesangium,
but this is an expected finding in a dog of this age. We do not
see proteinaceous fluid in the tubules or collecting ducts. The
proximal convoluted tubules are tightly packed and there is no evidence
of tubular necrosis, or interstitial inflammation or fibrosis.
In the liver, there are scattered lipogranulomas present, but not above
what we would expect to see in a dog of this age. There are low
numbers of lymphocytes and plasma cells within portal tracts. The
liver is otherwise normal.
The spleen has a few nodular areas of intense congestion and
subcapsular hemorrhage. There are large numbers of
hemosiderin-laden macrophages in these regions, suggesting that the
congestion has been present for some time. A few hematopoietic
cells can be seen.
The pancreas is normal.
The lesions are similar in each of the multiple sections of skin
examined. In all, there is a dense lichenoid band of inflammatory cells
at the dermoepidermal junction which, in several areas, obscures the
interface. These infiltrates are composed mostly of lymphocytes
accompanied by fewer plasma cells. Lymphocytes infiltrate into
the lower levels of the epithelium where we find occasional hydropic
swelling and apoptosis of basal keratinocytes. This is a
depigmenting lesion with large melanomacrophages scattered around
superficial blood vessels.
In some areas, there is "squamatization" of the epithelium with loss of
the basal layer and thinning of the epithelium. The surface is
covered by a thickened layer of keratin with serum accumulating between
keratin lamellae. Significant cellular crusting is not evident and
there is no indication of an acantholytic process.
In one of the sections from the nose, there is an area of deep
ulceration where we do find a few neutrophils within the exudates.
In the biopsy from the vulvar skin, we see, in addition to patchy
interface lesions as seen in the nose areas of pyoderma. These
consist of areas of epidermal spongiosis, pustule formation and
crusting with lesions of Europhilic folliculate and furunculosis.
Many of the inflamed hair follicles contain bacteria and some
have ruptured. The free hair and keratin and bacteria are
surrounded by numerous neutrophils and macrophages.
DIAGNOSIS:
1. LYPMPHOPLASMACYTIC AND EOSINOPHILIC GASTRITIS
2. NORMAL SMALL INTESTINE AND COLON
3. NORMAL KIDNEY, LIVER, SPLEEN AND PANCREAS
4. LYMPHOCYTIC INTERFACE NASAL AND
PERICULVAR DERMATITIS; MOST CONSISTENT WITH CHRONIC
LUPUS
ERYTHEMATOSUS
5. (ONE SECTION OF VULVAR SKIN) AREAS OF MUCOCUTANEOUS PYODERMA
6. LESIONS OF DEEP PYODERMA IN VULVAR SKIN SECTIONS
Comment:
There is evidence of chronic inflammation in the stomach, consistent
with inflammatory bowel disease, but we do not see inflammation in the
remainder of the gastrointestinal tract. The classification of
this lesion is challenging. Most institutions would probably
classify this lesion as lymphoplasmacytic IBD, since the predominant
cell type is the plasma cell. However, we would classify this lesion as
eosinophilic IBD, since although eosinophils are present in lower
number, we believe that their presence is
more significant since these cells have a short half life in tissues,
and lymphocytes and plasma cells will persist in tissues for longer and
thus will accumulate over time.
The sections from the skin of the vulva and the nose (most strikingly
in the nose) reveal lesions of discoid lupus erythematosus. In
the vulvar skin, there is also evidence of chronic but active deep
pyoderma.